Hong, Soon-Wooe and Yung-Chil, Hah (Dept. of Botany, Seoul National University, Seoul, Kerea.) A compantive investigation of free amino acids in healthy and virus diseased Chinese date tree. Kor. Jour. Bot. 4 (1)9~12 1961 : A comparative investigation of free amino acids content in healthy check and virus diseased leaves of Chinese date tree, Zizyphus jujuqa Mill var. inermis Rhed, was carried out by authors throughout the growing season of 1959 and 1960 from June to October.
The methods of qualitative analysis of free amino acids applied in this experiment is followed by Moore and Stein.^(1.2). free amino acids determined in this experiment are shown in Fig. 1 and Table 1. As the figure and the table are shown, three more amino acids such as glutarmine, asparagine and histidine are detected in the diseased material. The additiolal amino acids which are known as diamines in diseased leaves are conspicuous. It is presumed that the diamine might be increased by the self-reproduoion of the virus in cooporation with certain enzymes which are carrying out the protein metabolism in the host protoplast in contrast with the healthy checks which is carrying out normal protein metabolism,^(3.4.5). From the histological point of view, the facts of phloem degeneration or necrosis in diseased leaves, it seems to interrupt to move free amino acids from roots to leaves and it possibly takes place an excessive productitn of NH_3 which is diaminated by the metabolism of nitrogen compounds in such conditioned leaves.^(6.8) Therefore, it is also presumed that additional diamino acids are accumulated in diseased leaves.
There are no change of amino acids in both materials of this plant throughout the growing season qualitatively, and this result agrees with the paper of Knight.^9
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